Introduction
The Bioperfectus Viral Nucleic Acid Isolation Kit (Silica-Based Spin Column) was specially developed for the efficient purification of viral RNA and DNA from cell-free samples, such as serum, plasma, urine, body fluids, and supernatant from virus-infected cell cultures.
The sample is lysed by incubation with the Lysis Solution, which inactivates RNases, ensuring the protection of viral nucleic acids against degradation. Next, the lysed sample is transferred to a spin column, where the released nucleic acids immediately bind to the silica membrane in the presence of the Binding Buffer.
Remaining impurities are removed during three washing steps with Washing Buffer, while the pure nucleic acids remain bound to the membrane. Finally, the purified viral nucleic acids are eluted with the Elution Buffer. The obtained material is ready for use in downstream molecular biology applications.
Features
High purity and concentration
Fast: approximately 20 minutes per sample
Easy to use: the entire process involves only four steps
| Sample type | serum, plasma, urine, body fluids, and supernatant from virus-infected cell cultures |
| Sample volume | 200 μL |
| Analytes | DNA / RNA |
| Recovery | ≥90% |
| Elution volume | 50 μL |
| Technology | silica technology |
| Processing | manual |
| Storage temperature | room temperature |
| Transport temperature | room temperature |
NMPA, CE, FDA Listed, ANVISA
