The Hepatitis B Virus (HBV) Real-Time PCR Kit (RUO) is intended for the in vitro quantitative detection of Hepatitis B virus (HBV) DNA in serum and plasma samples. The kit was primarily developed for patients with Hepatitis B undergoing antiviral treatment and for patients with suspected HBV infection, allowing for the assessment of treatment response and efficacy by monitoring baseline levels and variations of HBV DNA in the patient’s blood.
HBV is the causative agent of Hepatitis B and belongs to the Hepadnaviridae family. It possesses partially double-stranded circular DNA with a genomic length of approximately 3.2 kb. Hepatitis B is a blood-borne disease, spread primarily through contact with contaminated blood (such as unsafe injections), childbirth (vertical transmission from mother to child), and sexual contact.
HBV can infect individuals year-round, without a fixed period of peak prevalence, usually occurring sporadically. In recent years, the incidence of Hepatitis B has increased significantly. HBV primarily causes inflammatory liver lesions and can affect other organs. In some cases, the infection can progress to cirrhosis or liver cancer.
This kit utilizes the quantitative fluorescent PCR technique to detect HBV concentration, providing conclusive evidence for the diagnosis of infection. It also assists in diagnostic confirmation, the definition of therapeutic indications, and the assessment of antiviral treatment efficacy.
LOD: 5 UI/mL
Internal Control: Monitors the efficiency of DNA extraction and PCR amplification of the samples
Standards: 4 quantitative reference standards for HBV
Sample Type: Serum or plasma
Packaging: Available in smaller packages: 24 tests/kit and 48 tests/kit
Genotype Coverage: Detection of HBV genotypes B, C, and D
ABI 7500 Real Time PCR System
QuantStudio™ 5 Real Time PCR System
Bioperfectus STC-96A / STC-96A PLUS Real Time PCR System
Roche LightCycler 480 PCR System
| Detection Limit (DL) |
5 UI/mL |
| Sample Input Volume |
30 µL |
| Storage | -20°C ± 5°C |
| Validity | 12 months |
