Vibrio cholerae Real-Time PCR Kit

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For Research Use Only (RUO)

Introduction

Vibrio cholerae is a comma-shaped, Gram-negative bacterium. It is the causative agent of cholera in humans, an infection that affects the upper small intestine. Transmission occurs primarily through contaminated water or food.
Individuals infected with cholera experience intense watery diarrhea and vomiting, caused by the cholera toxin produced by the bacteria. Many cases are potentially fatal, as diarrhea and vomiting can cause rapid dehydration and severe electrolyte loss. Occasional outbreaks and pandemics caused by Vibrio cholerae show that cholera remains a global threat to public health.

The BioPerfectus Vibrio cholerae Real-Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, intended for the detection of Vibrio cholerae O1, Vibrio cholerae O139, and total Vibrio cholerae DNA. Samples can be obtained from human feces.

Features

  • Multiplex PCR: Detects and differentiates Vibrio cholerae O1, O139, and total

  • Reliable: Limit of detection of 5 copies/reaction ($10^3$ copies/mL) with internal control

  • Efficient: 62 minutes to process 96 samples

  • User-friendly: Automatic interpretation of results with the STC real-time PCR system

Parameters

Target Vibrio cholerae – serogroups O1 and O139
LOD 5 copies/reaction ($10^3$ copies/mL)
Assay Time 62 minutes
Storage Temperature -20±5℃
Validity 12 months
Sample Input Volume

200 μL

Sample Type Human feces

 

CE

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